Huiyan Li, University of Guelph

Research

Early Career Researchers

Latest papers

Development of 3D printed capillaric circuit interfacing with silver nanoparticle embedded membrane for simultaneous analysis of duplex samples via fluorescence sandwich immunoassays.
Analytical and bioanalytical chemistry · 2026 · senior author
Development of a rapid calcium testing strip with metal-enhanced fluorescence for early detection of hypocalcemia in dairy cattle.
Mikrochimica acta · 2026 · senior author
Applications of metal enhanced fluorescence for the detection and quantification of extracellular vesicles.
Colloids and surfaces. B, Biointerfaces · 2026 · senior author

All 30 publications

Latest funding

Investigating Canadian milk-derived extracellular vesicles' impacts on human skin cells
NSERC · 2025 · Principal investigator
$109,092
Discovery of extracellular vesicle markers for predicting the metastatic potential of osteosarcoma
CIHR · 2024 · Nominated PI
$65,000
Engineering Highly Sensitive Bioassay Platforms with 3D Metal-Nanomaterial-Enhanced Fluorescence
NSERC · 2024 · Principal investigator
$12,500

All 10 grants and awards

30 publications.

01

Development of 3D printed capillaric circuit interfacing with silver nanoparticle embedded membrane for simultaneous analysis of duplex samples via fluorescence sandwich immunoassays.

Goodrum R, Li H

Analytical and bioanalytical chemistry2026Senior author

DOI: 10.1007/s00216-026-06636-6 PMID: 42362793

02

Development of a rapid calcium testing strip with metal-enhanced fluorescence for early detection of hypocalcemia in dairy cattle.

Miller L, Li H

Mikrochimica acta2026Senior author

DOI: 10.1007/s00604-026-08089-1 PMID: 42059954

03

Applications of metal enhanced fluorescence for the detection and quantification of extracellular vesicles.

Walker I, Li H

Colloids and surfaces. B, Biointerfaces2026Senior author

DOI: 10.1016/j.colsurfb.2025.115385 PMID: 41421261

04

Microprinted Epoxysilane Arrays for Conducting Microarray-Based Bioassays.

Aggarwal RT, Abdelrahman A, Herver Cruz MR, Patel S, Li H

Biotechnology journal2026Senior author

DOI: 10.1002/biot.70215 PMID: 41852339

05

Extracellular vesicle-induced silver-nanoparticle aggregation (EVISA) assay for rapid measurement of EV concentration.

Cook K, Vega NDC, Li H

Mikrochimica acta2026Senior author

DOI: 10.1007/s00604-026-07928-5 PMID: 41741789

06

Advancing Cancer Diagnostics: Technologies for Quantifying the Particle Concentration of Extracellular Vesicles in Complex Biological Samples.

Ströhle G, Misra M, Li H

Advanced healthcare materials2026Senior author

DOI: 10.1002/adhm.202503002 PMID: 41251186

07

The infection-exclusive proteome of murine extracellular vesicles defines Klebsiella pneumoniae-induced immune response signatures.

Honeywell E, Ball B, Sukumaran A, Muselius B, Ströhle G, Li H, Geddes-McAlister J

Canadian journal of microbiology2026

DOI: 10.1139/cjm-2025-0043 PMID: 41549367

08

Development of a silver nanoparticle embedded membrane platform for highly sensitive and multiplexed detection of extracellular vesicle proteins.

Goodrum R, Li H

Nanotechnology2025Senior author

DOI: 10.1088/1361-6528/ae2516 PMID: 41308201

09

Investigating the Impact of Various 2D and 3D Cell Culture Platforms on the Production of Extracellular Vesicles.

Cook K, Ströhle G, Li H

ACS applied bio materials2025Senior author

DOI: 10.1021/acsabm.5c01171 PMID: 40873241

10

Interfacial Dynamics in the Fabrication of Various Concave Hydrogel Discs for Enhanced Biosensing.

Bakhshayesh AG, Cook K, Li H

Polymers2025Senior author

DOI: 10.3390/polym17172341 PMID: 40942260

Investigating Canadian milk-derived extracellular vesicles' impacts on human skin cells

Principal investigatorNSERC2025Advantage

Principal investigators: Li, Huiyan

Keywords: Biomedical engineering

$109,092

Discovery of extracellular vesicle markers for predicting the metastatic potential of osteosarcoma

Nominated PICIHR2024CIHR - ICR / Cancer Research Society Partnership - Research Grants

Principal investigators: Li, Huiyan

Keywords: Early Career Researchers

$65,000

Engineering Highly Sensitive Bioassay Platforms with 3D Metal-Nanomaterial-Enhanced Fluorescence

Principal investigatorNSERC2024Discovery Launch Supplement

Principal investigators: Li, Huiyan

Keywords: bioassay development; hydrogel; nanomaterial; bioassay sensitivity; extracellular vesicles; proteins; multiplexing capability; metal enhanced fluorescence; biosensing

$12,500

A hydrogel-based bacteria concentrator for rapid highly sensitive detection of Legionella in water

Principal investigatorNSERC2024Alliance Grants

Principal investigators: Li, Huiyan H

Keywords: assay sensitivity; bacteria concentration; hydrogel; lateral flow assay; legionella detection; turnaround time; water samples

$30,000

Compartmentalized Linker Array (CLA) for next-generation multiplexed bioassays

Principal investigatorNSERC2024Idea to Innovation

Principal investigators: Li, Huiyan H

Keywords: assay validation; customizable; extracellular vesicles; high specificity; low-cost; mass manufacture; multiplexed bioassay; protein quantification; scalable; simple-to-implement

$125,000

Developing a microfluidic system for sensitive, rapid, multiplexed protein quantification using a gold-nanoparticle-embedded membrane platform

Principal investigatorNSERC2024Alliance Grants

Principal investigators: Li, Huiyan H

Keywords: assay sensitivity; compartments; gold nanoparticles; membrane; microfluidic system; multiplexed immunoassays; rapid immunoassays; sample-to-result time

$25,000

Engineering Highly Sensitive Bioassay Platforms with 3D Metal-Nanomaterial-Enhanced Fluorescence

Principal investigatorNSERC2024Discovery Grants Program - Individual

Principal investigators: Li, Huiyan

Keywords: bioassay development; hydrogel; nanomaterial; bioassay sensitivity; extracellular vesicles; proteins; multiplexing capability; metal enhanced fluorescence; biosensing

$45,000

L2M NSERC - GEM: Gold nanoparticle Embedded paper Membrane for highly sensitive immunoassays

Principal investigatorNSERC2023Idea to Innovation

Principal investigators: Li, Huiyan H

$20,000

Convenient and low-cost methods and devices for performing reproducible multiplexed bioassays

Principal investigatorNSERC2022Idea to Innovation

Principal investigators: Li, Huiyan

$20,000

Development of a high-yield and high-purity isolation kit for extracting extracellular vesicles from complex biological samples

Principal investigatorNSERC2021Alliance Grants

Principal investigators: Li, Huiyan

$86,670

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Jennifer Geddes-McAlister

Molecular and Cellular Biology

1 shared papers, latest 2026

Write to this professor

A short, specific email works best. This draft uses one of their recent papers; replace the parts in brackets with your own details before sending.

Applying for

Paper to mentionMention their NSERC grant from 2025

Subject: Prospective PhD student: Early Career Researchers Dear Professor Li, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "Development of 3D printed capillaric circuit interfacing with silver nanoparticle embedded membrane for simultaneous analysis of duplex samples via fluorescence sandwich immunoassays." (2026), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your NSERC-funded project "Investigating Canadian milk-derived extracellular vesicles' impacts on human skin cells" (2025), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]