Gary Brouhard, McGill University

Research

Microtubule Doublecortin Biochemistry Biophysics Cell Biology Kinesin Microscopy Tau

Latest papers

Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.
Current biology : CB · 2025
Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.
bioRxiv : the preprint server for biology · 2024
Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C-terminal domain.
eLife · 2022

All 7 publications

Latest funding

Regulation of axonal transport and cytoskeletal architecture by tau
CIHR · 2026 · Co-investigator
$1,262,250
The Doublecortin Family in Development and Disease
CIHR · 2021 · Nominated PI
$960,075
Controlling cellular and organismal microenvironment using light
NSERC · 2021 · Co-investigator
$150,000

All 13 grants and awards

7 publications.

01

Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.

Legal T, Joachimiak E, Parra M, Peng W, Tam A, Black C, Guha M, Nguyen CA, Ghanaeian A, Valente-Paterno M, Brouhard G, Gaertig J, Wloga D, Bui KH

Current biology : CB2025

DOI: 10.1016/j.cub.2025.06.020 PMID: 40651469

02

Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.

Legal T, Joachimiak E, Parra M, Peng W, Tam A, Black C, Valente-Paterno M, Brouhard G, Gaertig J, Wloga D, Bui KH

bioRxiv : the preprint server for biology2024

DOI: 10.1101/2024.12.02.626492 PMID: 39677611

03

Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C-terminal domain.

Rafiei A, Cruz Tetlalmatzi S, Edrington CH, Lee L, Crowder DA, Saltzberg DJ, Sali A, Brouhard G, Schriemer DC

eLife2022

DOI: 10.7554/elife.66975 PMID: 35485925

04

Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding.

Khan N, Pelletier D, McAlear TS, Croteau N, Veyron S, Bayne AN, Black C, Ichikawa M, Khalifa AAZ, Chaaban S, Kurinov I, Brouhard G, Bechstedt S, Bui KH, Trempe JF

Structure (London, England : 1993)2021

DOI: 10.1016/j.str.2021.01.001 PMID: 33529594

05

Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.

Kowanda M, Bergalet J, Wieczorek M, Brouhard G, Lécuyer É, Lasko P

Biology open2016

DOI: 10.1242/bio.019638 PMID: 27422905

06

Collapsin response mediator protein 4 regulates growth cone dynamics through the actin and microtubule cytoskeleton.

Khazaei MR, Girouard MP, Alchini R, Ong Tone S, Shimada T, Bechstedt S, Cowan M, Guillet D, Wiseman PW, Brouhard G, Cloutier JF, Fournier AE

The Journal of biological chemistry2014

DOI: 10.1074/jbc.m114.570440 PMID: 25225289

07

Microtubules: sizing up the GTP cap.

Brouhard G, Sept D

Current biology : CB2012First author

DOI: 10.1016/j.cub.2012.07.050 PMID: 23017994

Regulation of axonal transport and cytoskeletal architecture by tau

Co-investigatorCIHR2026Project Grant

Principal investigators: Hendricks, Adam G

Keywords: Alzheimer'S Disease; Dynein; Ipscs; Kinesin; Microtubule; Neurodegeneration; Single-Molecule Biophysics; Tau

$1,262,250

The Doublecortin Family in Development and Disease

Nominated PICIHR2021Project Grant

Principal investigators: Brouhard, Gary J

Keywords: Doublecortin; Microtubule

$960,075

Controlling cellular and organismal microenvironment using light

Co-investigatorNSERC2021Research Tools and Instruments

Principal investigators: Hayer, Arnold

$150,000

Non-mammalian Microtubules and Microtubule-Associated Proteins

Principal investigatorNSERC2020Discovery Grants Program - Individual

Principal investigators: Brouhard, Gary

$300,000

Bioengineering Facility for UV Structured Substrate Patterning

Co-investigatorNSERC2018Research Tools and Instruments

Principal investigators: Ehrlicher, Allen

$150,000

Regulation of Microtubule Activity in Neurons

Nominated PICIHR2016Project Grant

Principal investigators: Brouhard, Gary J

Keywords: Biochemistry; Biophysics; Cell Biology; Doublecortin; Kinesin; Microscopy; Microtubule; Tau

$760,680

The Cell Biology and Biochemistry of Microtubule Nucleation

Nominated PICIHR2014Operating Grant

Principal investigators: Brouhard, Gary J

Keywords: Centrosome; Cytoskeleton; Microcephaly; Microtubule; Microtubule-Associated Protein; Mitosis; Nucleation; Tpx2

$757,164

The functional significance of microtubule quaternary structure

Principal investigatorNSERC2014Discovery Grants Program - Individual

Principal investigators: Brouhard, Gary

$175,000

Super-resolution microscope based on stochastic optical reconstruction microscopy (STORM)

Principal investigatorNSERC2011Research Tools and Instruments - Category 1 (<$150,000)

Principal investigators: Brouhard, Gary

$132,600

Control of Microtubule Architecture by Doublecortin

Nominated PICIHR2011CIHR New Investigator

Principal investigators: Brouhard, Gary J

Keywords: Biophysics; Cortex; Doublecortin; Lissencephaly; Microtubulue; Neuron; Nucleation; Single Molecule

$300,000

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Susanne Bechstedt

Anatomy and Cell Biology

2 shared papers, latest 2021

Write to this professor

A short, specific email works best. This draft uses one of their recent papers; replace the parts in brackets with your own details before sending.

Applying for

Paper to mentionMention their CIHR grant from 2021

Subject: Prospective PhD student: Microtubule Dear Professor Brouhard, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1." (2025), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your CIHR-funded project "The Doublecortin Family in Development and Disease" (2021), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]