Pascale Legault, Université de Montréal

Research

Riboproteomics Rna-Protein Interactions Alpha-Synuclein Control Of Gene Expression Ipsc Lines Microrna Maturation Micrornas Mouse Models

Latest papers

PTPRN2 hypomethylation and PHB2-associated miR-153-3p maturation define dual epigenetic features linked to symptom variability in Myalgic encephalomyelitis.
Journal of translational medicine · 2026
Correction: Substrate promiscuity of Dicer toward precursors of the let-7 family and their 3'-end modifications.
Cellular and molecular life sciences : CMLS · 2025 · senior author
Substrate promiscuity of Dicer toward precursors of the let-7 family and their 3'-end modifications.
Cellular and molecular life sciences : CMLS · 2024 · senior author

All 12 publications

Latest funding

23rd RiboClub Annual Meeting
CIHR · 2022 · Co-investigator
$10,000
Maturation of microRNAs targeting alpha-synuclein
CIHR · 2022 · Nominated PI
$1,021,275
Maturation of let-7 miRNAs
NSERC · 2020 · Principal investigator
$300,000

All 20 grants and awards

12 publications.

01

PTPRN2 hypomethylation and PHB2-associated miR-153-3p maturation define dual epigenetic features linked to symptom variability in Myalgic encephalomyelitis.

Chalder L, Elremaly W, Li D, Fang Y, Caraus I, Leveau C, Elbakry M, Franco A, Godbout C, Di Tomasso G, Nepotchatykh E, Rostami-Afshari B, Gimenez M, Legault P, Moreau A

Journal of translational medicine2026

DOI: 10.1186/s12967-026-08162-6 PMID: 42010606

02

Correction: Substrate promiscuity of Dicer toward precursors of the let-7 family and their 3'-end modifications.

Dadhwal G, Samy H, Bouvette J, El-Azzouzi F, Dagenais P, Legault P

Cellular and molecular life sciences : CMLS2025Senior author

DOI: 10.1007/s00018-025-05795-6 PMID: 40576821

03

Substrate promiscuity of Dicer toward precursors of the let-7 family and their 3'-end modifications.

Dadhwal G, Samy Saad H, Bouvette J, El-Azzouzi F, Dagenais P, Legault P

Cellular and molecular life sciences : CMLS2024Senior author

DOI: 10.1007/s00018-023-05090-2 PMID: 38261114

04

An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme.

Dagenais P, Desjardins G, Legault P

Nucleic acids research2021Senior author

DOI: 10.1093/nar/gkab963 PMID: 34718697

05

In Vitro Selection of Varkud Satellite Ribozyme Variants that Cleave a Modified Stem-Loop Substrate.

Dagenais P, Legault P

Methods in molecular biology (Clifton, N.J.)2021Senior author

DOI: 10.1007/978-1-0716-0716-9_5 PMID: 32712915

06

High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension.

Bouvette J, Korkut DN, Fouillen A, Amellah S, Nanci A, Durocher Y, Omichinski JG, Legault P

BMC biotechnology2018Senior author

DOI: 10.1186/s12896-018-0485-3 PMID: 30522464

07

ARiBo pull-down for riboproteomic studies based on label-free quantitative mass spectrometry.

Di Tomasso G, Miller Jenkins LM, Legault P

RNA (New York, N.Y.)2016Senior author

DOI: 10.1261/rna.057513.116 PMID: 27659051

08

Rational engineering of the Neurospora VS ribozyme to allow substrate recognition via different kissing-loop interactions.

Lacroix-Labonté J, Girard N, Dagenais P, Legault P

Nucleic acids research2016Senior author

DOI: 10.1093/nar/gkw401 PMID: 27166370

09

Affinity purification of in vitro transcribed RNA with homogeneous ends using a 3'-ARiBo tag.

Di Tomasso G, Salvail-Lacoste A, Bouvette J, Omichinski JG, Legault P

Methods in enzymology2014Senior author

DOI: 10.1016/b978-0-12-801122-5.00003-9 PMID: 25432744

10

Preparative separation of ribonucleoside monophosphates by ion-pair reverse-phase HPLC.

Dagenais P, Legault P

Methods in molecular biology (Clifton, N.J.)2012Senior author

DOI: 10.1007/978-1-62703-113-4_18 PMID: 23065566

23rd RiboClub Annual Meeting

Co-investigatorCIHR2022Planning and Dissemination Grant - Institute Community Support

Principal investigators: Scott, Michelle

Keywords: Deregulation In Disease; Gene Expression Regulation; Regulatory Rna; Rna Biology; Rna Complexes; Rna Delivery; Rna In Biotechnology; Rna Interactions; Rna Structure; Transcriptomics

$10,000

Maturation of microRNAs targeting alpha-synuclein

Nominated PICIHR2022Project Grant

Principal investigators: Legault, Pascale

Keywords: Alpha-Synuclein; Control Of Gene Expression; Ipsc Lines; Microrna Maturation; Micrornas; Mouse Models; Primary Dopaminergic Neurons; Riboproteomics; Rna-Protein Interactions; Transcriptomics

$1,021,275

Maturation of let-7 miRNAs

Principal investigatorNSERC2020Discovery Grants Program - Individual

Principal investigators: Legault, Pascale

$300,000

Macromolecular interaction analysis using a surface plasmon resonance biosensor

Co-investigatorNSERC2018Research Tools and Instruments

Principal investigators: Pascal, John

$148,525

Structural and Engineering Studies of Ribozymes and Riboswitches

Nominated PICIHR2016Project Grant

Principal investigators: Legault, Pascale

Keywords: Antibiotics; Enzymology; Ligand Binding; Nmr Spectroscopy; Riboswitches; Ribozyme; Rna Engineering; Rna Targeting; Structural Biology; X-Ray Crystallography

$875,355

Characterization of macromolecular complexes regulating let-7 microRNA biogenesis

Principal investigatorNSERC2015Discovery Grants Program - Individual

Principal investigators: Legault, Pascale

$225,000

The ARiBo technology for isolation of ribonucleoprotein complexes

Nominated PICIHR2012Proof of Principle Program - Phase I

Principal investigators: Legault, Pascale

Keywords: Activatable Ribozymes; Affinity Purification Of Rna; Isolation Of Rnp Complexes; Lambda Boxb Rna; Lambda N Peptide; Micro Rna; Ribonucleoprotein Complexes; Riboproteomics; Rna-Protein Interactions; Tagged Rna

$145,412

Low-volume ITC for investigating biomolecular interactions

Co-investigatorNSERC2012Research Tools and Instruments - Category 1 (<$150,000)

Principal investigators: Omichinski, James

$130,099

A molecular imager for Quantitative biochemistry

Principal investigatorNSERC2011Research Tools and Instruments - Category 1 (<$150,000)

Principal investigators: Legault, Pascale

$140,400

Macromolecular crystallization facility

Co-investigatorNSERC2010Research Tools and Instruments - Category 1 (<$150,000)

Principal investigators: Sygusch, Jurgen

$149,884

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Biochimie et médecine moléculaire
Stomatology
Other

Co-authors at Université de Montréal, colored by department. Thicker lines mean more shared papers; select anyone to open their profile and their own map.

Alain Moreau

Stomatology

1 shared papers, latest 2026

Alain Moreau

Faculty

1 shared papers, latest 2026

Write to this professor

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Applying for

Paper to mentionMention their CIHR grant from 2022

Subject: Prospective PhD student: Riboproteomics Dear Professor Legault, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "PTPRN2 hypomethylation and PHB2-associated miR-153-3p maturation define dual epigenetic features linked to symptom variability in Myalgic encephalomyelitis." (2026), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your CIHR-funded project "Maturation of microRNAs targeting alpha-synuclein" (2022), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]