Christina Guzzo, University of Toronto

Research

Flow Virometry Hiv Envelope Glycoprotein (Gp120)Peripheral Blood Mononuclear Cells (Pbmc)Virion-Incorporated Proteins Cd4+ T Cells Flow Cytometry Virus Attachment Clinical And Lab-Adapted Hiv Isolates

Latest papers

Applying intravirion staining to discern extracellular vesicles from viruses with flow virometry.
Virology · 2026 · senior author
Comparing methods to detect cellular proteins on the surface of HIV-1 virions.
Journal of virological methods · 2025 · senior author
Flow virometry: recent advancements, best practices, and future frontiers.
Journal of virology · 2025 · senior author

All 12 publications

Latest funding

Investigating the topology of viral surfaces to inform improved therapeutics & vaccines.
CIHR · 2023 · Supervisor
$270,000
Single Virus Particle Analyses
NSERC · 2023 · Principal investigator
$120,000
Using unique methods in flow virometry to discover new proteins on the HIV surface and their impact on HIV infection.
CIHR · 2023 · Nominated PI
$300,000

All 9 grants and awards

12 publications.

01

Applying intravirion staining to discern extracellular vesicles from viruses with flow virometry.

Chaphekar D, Rajakumar RC, Persaud AT, Fernandes C, Farheen A, Guzzo C

Virology2026Senior author

DOI: 10.1016/j.virol.2026.111081 PMID: 42743719

02

Comparing methods to detect cellular proteins on the surface of HIV-1 virions.

Chaphekar D, Fernandes C, Persaud AT, Guzzo C

Journal of virological methods2025Senior author

DOI: 10.1016/j.jviromet.2024.115096 PMID: 39647666

03

Flow virometry: recent advancements, best practices, and future frontiers.

Fernandes C, Persaud AT, Chaphekhar D, Burnie J, Belanger C, Tang VA, Guzzo C

Journal of virology2025Senior author

DOI: 10.1128/jvi.01717-24 PMID: 39868829

04

Applying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences Across HIV Model Systems.

Burnie J, Fernandes C, Patel A, Persaud AT, Chaphekar D, Wei D, Lee TKH, Tang VA, Cicala C, Arthos J, Guzzo C

Viruses2024Senior author

DOI: 10.3390/v16060935 PMID: 38932227

05

Virion-incorporated CD14 enables HIV-1 to bind LPS and initiate TLR4 signaling in immune cells.

Persaud AT, Khela J, Fernandes C, Chaphekar D, Burnie J, Tang VA, Colpitts CC, Guzzo C

Journal of virology2024Senior author

DOI: 10.1128/jvi.00363-24 PMID: 38661384

06

Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay.

Burnie J, Fernandes C, Chaphekar D, Wei D, Ahmed S, Persaud AT, Khader N, Cicala C, Arthos J, Tang VA, Guzzo C

Scientific reports2023Senior author

DOI: 10.1038/s41598-023-50365-0 PMID: 38155248

07

P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is incorporated into clinical HIV-1 isolates and can mediate virus capture and subsequent transfer to permissive cells.

Burnie J, Persaud AT, Thaya L, Liu Q, Miao H, Grabinsky S, Norouzi V, Lusso P, Tang VA, Guzzo C

Retrovirology2022Senior author

DOI: 10.1186/s12977-022-00593-5 PMID: 35597982

08

Human monocytes store and secrete preformed CCL5, independent of de novo protein synthesis.

Persaud AT, Bennett SA, Thaya L, Burnie J, Guzzo C

Journal of leukocyte biology2022Senior author

DOI: 10.1002/jlb.3a0820-522rr PMID: 34114669

09

A UV-LED module that is highly effective at inactivating human coronaviruses and HIV-1.

Persaud AT, Burnie J, Thaya L, DSouza L, Martin S, Guzzo C

Virology journal2022Senior author

DOI: 10.1186/s12985-022-01754-w PMID: 35144624

10

Engineering pan-HIV-1 neutralization potency through multispecific antibody avidity.

Rujas E, Cui H, Burnie J, Aschner CB, Zhao T, Insausti S, Muthuraman K, Semesi A, Ophel J, Nieva JL, Seaman MS, Guzzo C, Treanor B, Julien JP

Proceedings of the National Academy of Sciences of the United States of America2022

DOI: 10.1073/pnas.2112887119 PMID: 35064083

Investigating the topology of viral surfaces to inform improved therapeutics & vaccines.

SupervisorCIHR2023CIHR REDI Early Career Transition Award - HIV/AIDS and STBBI

Principal investigators: Burnie, Jonathan D

Keywords: Coronaviruses; Emerging Pathogens; Endemic Coronaviruses; Flow Virometry; Hiv; Pandemic Preparedness; Therapeutics; Vaccines; Viral Immunology; Viral Pathogens

$270,000

Single Virus Particle Analyses

Principal investigatorNSERC2023Canada Research Chairs

Principal investigators: Guzzo, Christina

Keywords: Human immunodeficiency virus (HIV); Virion-incorporated proteins; Virus sorting; Viral proteomics; Viral diagnostics; Nanoparticles; Nanoscale cytometry

$120,000

Using unique methods in flow virometry to discover new proteins on the HIV surface and their impact on HIV infection.

Nominated PICIHR2023Project Grant - PA: HIV/AIDS and STBBI Multi-Year Grant

Principal investigators: Guzzo, Christina R

Keywords: Cd4+ T Cells; Flow Cytometry; Flow Virometry; Hiv Envelope Glycoprotein (Gp120); Hiv-1; Humanized Mice; Peripheral Blood Mononuclear Cells (Pbmc); Virion-Incorporated Proteins; Virus Attachment; Virus Capture Assay

$300,000

Using Unique Methods in Flow Virometry to Identify and Study Proteins on the HIV Surface.

Nominated PICIHR2022Project Grant - PA: HIV/AIDS and STBBI Multi-Year Grant

Principal investigators: Guzzo, Christina R

Keywords: Cd4+ T Cels; Clinical And Lab-Adapted Hiv Isolates; Flow Cytometry; Flow Virometry; Hiv Envelope Glycoprotein (Gp120); Human Immunodeficiency Virus - Type 1 (Hiv-1); Peripheral Blood Mononuclear Cells (Pbmc); Virion-Incorporated Proteins; Virus Attachment; Virus Capture

$300,000

Rapid development of automated antiviral lighting systems for use as a COVID-19 prevention tool_x000d_

Principal investigatorNSERC2020Alliance Grants

Principal investigators: Guzzo, Christina

$50,000

The incorporation of human proteins into the external HIV envelope: using novel tools to phenotype viral surfaces and identify new antiviral targets.

Nominated PICIHR2020Project Grant - Priority Announcement: HIV/AIDS and STBBI

Principal investigators: Guzzo, Christina R

Keywords: Cd4+ T Cells; Clinical And Lab-Adapted Hiv Isolates; Flow Virometry; Hiv Envelope Glycoprotein (Gp120); Human Immunodeficiency Virus 1 (Hiv-1); Integrin Alpha 4 Beta 7; Peripheral Blood Mononuclear Cells (Pbmc); Virion-Incorporated Proteins; Virus Immunocapture; Virus Neutralization

$100,000

Investigation of the Role of Host Proteins in the HIV-1 Envelope

SupervisorCIHR2019Canada Graduate Scholarships - Michael Smith Foreign Study Supplements

Principal investigators: Burnie, Jonathan D

Keywords: Hiv; Investigation; Proteins; Role

$6,000

Novel Mechanisms of Cytokine Storage and Secretion

Principal investigatorNSERC2019Discovery Launch Supplement

Principal investigators: Guzzo, Christina

$12,500

Novel Mechanisms of Cytokine Storage and Secretion

Principal investigatorNSERC2019Discovery Grants Program - Individual

Principal investigators: Guzzo, Christina

$222,000

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Biological Sciences
Department of Biochemistry

Co-authors at University of Toronto, colored by department. Thicker lines mean more shared papers; select anyone to open their profile and their own map.

Bebhinn Treanor

Biological Sciences

1 shared papers, latest 2022

Jean-Philippe Julien

Department of Biochemistry

1 shared papers, latest 2022

Write to this professor

A short, specific email works best. This draft uses one of their recent papers; replace the parts in brackets with your own details before sending.

Applying for

Paper to mentionMention their NSERC grant from 2023

Subject: Prospective PhD student: Flow Virometry Dear Professor Guzzo, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "Applying intravirion staining to discern extracellular vesicles from viruses with flow virometry." (2026), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your NSERC-funded project "Single Virus Particle Analyses" (2023), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]