Daniel De Carvalho

Research

Immunotherapy Dna Methylation Colorectal Cancer Epigenetic Therapy Epigenetics Crc Cancer Stem Cells Adoptive Cell Therapy

Latest papers

Evolutionary dynamics of recurrent hepatocellular carcinoma under divergent immune selection pressures.
Frontiers in oncology · 2025
Cell-free DNA methylation-defined prognostic subgroups in small-cell lung cancer identified by leukocyte methylation subtraction.
iScience · 2022
Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities.
The Journal of cell biology · 2018

All 4 publications

Latest funding

Natural Killer Cell Killing Resistance Mechanisms in Acute Myeloid Leukemia Stem Cells
CIHR · 2025 · Supervisor
$35,000
Disruption of the SPT-ADA-GCN5-ACETYLTRANSFERASE (SAGA) complex in adoptive cell therapies to enhance killing capacity and long-term immune cells effector function
CIHR · 2024 · Nominated PI
$860,626
Liquid biopsy-based DNA methylation profiling for improved diagnosis and prognostication of primary and metastatic CNS tumors
CIHR · 2020 · Co-investigator
$1,147,500

All 19 grants and awards

4 publications.

01

Evolutionary dynamics of recurrent hepatocellular carcinoma under divergent immune selection pressures.

Chakravarthy A, Pasini E, Zhao X, To J, Shen SYR, Fischer S, Ghanekar A, Vogel A, Grant RC, Knox J, Sapisochin G, Gores GJ, De Carvalho D, Bhat M

Frontiers in oncology2025

DOI: 10.3389/fonc.2025.1537087 PMID: 40831927

02

Cell-free DNA methylation-defined prognostic subgroups in small-cell lung cancer identified by leukocyte methylation subtraction.

Ul Haq S, Schmid S, Aparnathi MK, Hueniken K, Zhan LJ, Sacdalan D, Li JJN, Meti N, Patel D, Cheng D, Philip V, Tsao MS, Cabanero M, de Carvalho D, Liu G, Bratman SV, Lok BH

iScience2022

DOI: 10.1016/j.isci.2022.105487 PMID: 36425756

03

Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities.

Casey AE, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, Cruickshank J, Shehata M, Drysdale E, Fang H, Kim H, Isserlin R, Bailey S, Medina T, Deblois G, Shiah YJ, Barsyte-Lovejoy D, Hofer S, Bader G, Lupien M, Arrowsmith C, Knapp S, De Carvalho D, Berman H, Boutros PC, Kislinger T, Khokha R

The Journal of cell biology2018

DOI: 10.1083/jcb.201804042 PMID: 29921600

04

Symmetrical Dose-Dependent DNA-Methylation Profiles in Children with Deletion or Duplication of 7q11.23.

Strong E, Butcher DT, Singhania R, Mervis CB, Morris CA, De Carvalho D, Weksberg R, Osborne LR

American journal of human genetics2015

DOI: 10.1016/j.ajhg.2015.05.019 PMID: 26166478

Natural Killer Cell Killing Resistance Mechanisms in Acute Myeloid Leukemia Stem Cells

SupervisorCIHR2025Cancer Research Society Doctoral Research Awards - ICR-ISI

Principal investigators: Wu, Taiyi

Keywords: Acute Myeloid Leukemia; Leukemia; Nk Cell Mediated Cytotoxicity; Nk Cells

$35,000

Disruption of the SPT-ADA-GCN5-ACETYLTRANSFERASE (SAGA) complex in adoptive cell therapies to enhance killing capacity and long-term immune cells effector function

Nominated PICIHR2024Project Grant

Principal investigators: De Carvalho, Daniel

Keywords: Adoptive Cell Therapy; Chimeric Antigen Receptor (Car) T Cells; Crispr-Cas9; Epigenetics; Genome Editing; Immunotherapy; Saga Complex; T Cells

$860,626

Liquid biopsy-based DNA methylation profiling for improved diagnosis and prognostication of primary and metastatic CNS tumors

Co-investigatorCIHR2020Project Grant

Principal investigators: Zadeh, Gelareh

Keywords: Biomarkers; Brain Metastasis; Cerebrospinal Fluid; Diagnosis; Dna Methylation; Genomics; Glioblastoma; Liquid Biopsy; Plasma; Prognosis

$1,147,500

Canada Research Chair - Tier 2

Nominated PICIHR2020Canada Research Chairs - CIHR Funded - Tier 2

Principal investigators: De Carvalho, Daniel

Keywords: Crc

$500,000

Mapping epigenomic makeup in colorectal cancer patient models to identify novel therapeutic options

Co-investigatorCIHR2020Project Grant

Principal investigators: Orouji, Elias

Keywords: Chromatin State; Colorectal Cancer; Epigenomics; Histone Modifications; Patient-Derived Organoids

$814,726

Lung cancer early detection and classification using methylome analysis of plasma cell free DNA

Co-investigatorCIHR2019Project Grant

Principal investigators: Bratman, Scott V; Liu, Geoffrey

Keywords: Cell-Free Dna; Ctdna; Diagnostics; Dna Methylation; Early Detection; Epigenomics; Liquid Biopsy; Lung Cancer; Machine Learning; Screening

$995,264

Viral mimicry tolerance as a mechanism of early tumor immune evasion

Nominated PICIHR2019Project Grant

Principal investigators: De Carvalho, Daniel

Keywords: Cancer; Dna Methylation; Epigenetics; Histone Methylation; Immunotherapy; Ovarian Cancer; Therapy Resistance

$868,276

Early detection of cancer in high-risk patients through profiling of circulating tumour DNA

Co-investigatorCIHR2018Project Grant

Principal investigators: Pugh, Trevor J; Kim, Raymond; Pollett, Aaron

Keywords: Circulating Tumour Dna; Epigenetics; Genomics; Hereditary Cancer Syndrome

$1,977,525

DNA methylation profiling in cell-free DNA: a non-invasive method to screen for pre-term birth

Co-investigatorCIHR2018Project Grant

Principal investigators: Hoffman, Michael M

Keywords: Cell-Free Dna; Machine Learning; Methylation Dna Immunoprecipitation (Medip)

$1,212,525

Overcoming epigenetic therapy resistance to augment immunotherapy in ovarian cancer

SupervisorCIHR2018CIHR Fellowship

Principal investigators: Ishak, Charles A

Keywords: Cancer; Clinical Trials; Dna Methylation; Epigenetics; Histone Methylation; Immunotherapy; Mouse Models; Ovarian Cancer; Therapy Resistance

$135,000

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Medical Biophysics
Department of Medical Biophysics
Pharmacology and Toxicology
Terrence Donnelly Centre for Cellular and Biomolecular Research
Epidemiology Division
Transplant Program
Cancer Research
Other

Co-authors at University of Toronto, colored by department. Thicker lines mean more shared papers; select anyone to open their profile and their own map.

Mamatha Bhat

Transplant Program

1 shared papers, latest 2025

Dalia Barsyte-Lovejoy

Pharmacology and Toxicology

1 shared papers, latest 2018

Anand Ghanekar

Faculty

1 shared papers, latest 2025

Gonzalo Sapisochin

Faculty

1 shared papers, latest 2025

Arndt Vogel

Faculty

1 shared papers, latest 2025

Geoffrey Liu

Epidemiology Division

1 shared papers, latest 2022

Thomas Kislinger

Department of Medical Biophysics

1 shared papers, latest 2018

Stefan Hofer

Plastic Surgery

1 shared papers, latest 2018

Rosanna Weksberg

Genetics and Genome Biology

1 shared papers, latest 2015

Rama Khokha

Cancer Research

1 shared papers, latest 2018

Cheryl Arrowsmith

Department of Medical Biophysics

1 shared papers, latest 2018

Mathieu Lupien

Medical Biophysics

1 shared papers, latest 2018

Gary Bader

Terrence Donnelly Centre for Cellular and Biomolecular Research

1 shared papers, latest 2018

Write to this professor

A short, specific email works best. This draft uses one of their recent papers; replace the parts in brackets with your own details before sending.

Applying for

Paper to mentionMention their CIHR grant from 2024

Subject: Prospective PhD student: Immunotherapy Dear Professor De Carvalho, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "Evolutionary dynamics of recurrent hepatocellular carcinoma under divergent immune selection pressures." (2025), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your CIHR-funded project "Disruption of the SPT-ADA-GCN5-ACETYLTRANSFERASE (SAGA) complex in adoptive cell therapies to enhance killing capacity and long-term immune cells effector function" (2024), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]