Mojca Mattiazzi Usaj, Toronto Metropolitan University

Research

high-content imaging interaction netrowks phenotype penetrance phenotypic heterogeneity quantitative cell biology single-cell analysis subcellular morphology systems biology

Latest papers

Vacuolar pH regulates clathrin-mediated endocytosis through TORC1 signaling during replicative aging.
The Journal of cell biology · 2025 · senior author
PIFiA: self-supervised approach for protein functional annotation from single-cell imaging data.
Molecular systems biology · 2024
From pixels to insights: Machine learning and deep learning for bioimage analysis.
BioEssays : news and reviews in molecular, cellular and developmental biology · 2024 · senior author

All 12 publications

Latest funding

A Dual Camera Acquisition-Spinning Disc Confocal Microscope System to Study Cellular Dynamics
NSERC · 2023 · Co-investigator
$150,000
Quantitative dissection of sources of phenotypic heterogeneity
NSERC · 2021 · Principal investigator
$164,000
Quantitative dissection of sources of phenotypic heterogeneity
NSERC · 2021 · Principal investigator
$12,500

All 3 grants and awards

12 publications.

01

Vacuolar pH regulates clathrin-mediated endocytosis through TORC1 signaling during replicative aging.

Antenor KG, Lee-Dadswell J, Salahshour N, Grishchenko N, Swaleh S, Makhija G, Spangaro A, Mattiazzi Usaj M

The Journal of cell biology2025Senior author

DOI: 10.1083/jcb.202412064 PMID: 40990464

02

PIFiA: self-supervised approach for protein functional annotation from single-cell imaging data.

Razdaibiedina A, Brechalov A, Friesen H, Mattiazzi Usaj M, Masinas MPD, Garadi Suresh H, Wang K, Boone C, Ba J, Andrews B

Molecular systems biology2024

DOI: 10.1038/s44320-024-00029-6 PMID: 38472305

03

From pixels to insights: Machine learning and deep learning for bioimage analysis.

Jan M, Spangaro A, Lenartowicz M, Mattiazzi Usaj M

BioEssays : news and reviews in molecular, cellular and developmental biology2024Senior author

DOI: 10.1002/bies.202300114 PMID: 38058114

04

Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.

Mattiazzi Usaj M, Yeung CHL, Friesen H, Boone C, Andrews BJ

Cell systems2021First author

DOI: 10.1016/j.cels.2021.05.010 PMID: 34139168

05

Systematic High-Content Screening of Fluorescently Tagged Yeast Double Mutant Strains.

Garadi Suresh H, Mattiazzi Usaj M

Methods in molecular biology (Clifton, N.J.)2021Senior author

DOI: 10.1007/978-1-0716-1740-3_3 PMID: 34590270

06

High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.

Mattiazzi Usaj M, Lo DS, Grys BT, Andrews BJ

Methods in molecular biology (Clifton, N.J.)2021First author

DOI: 10.1007/978-1-0716-1402-0_12 PMID: 34028720

07

Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

Mattiazzi Usaj M, Sahin N, Friesen H, Pons C, Usaj M, Masinas MPD, Shuteriqi E, Shkurin A, Aloy P, Morris Q, Boone C, Andrews BJ

Molecular systems biology2020First author

DOI: 10.15252/msb.20199243 PMID: 32064787

08

Systematic analysis of complex genetic interactions.

Kuzmin E, VanderSluis B, Wang W, Tan G, Deshpande R, Chen Y, Usaj M, Balint A, Mattiazzi Usaj M, van Leeuwen J, Koch EN, Pons C, Dagilis AJ, Pryszlak M, Wang ZY, Hanchard J, Riggi M, Xu K, Heydari H, San Luis BJ, Shuteriqi E, Zhu H, Van Dyk N, Sharifpoor S, Costanzo M, Loewith R, Caudy A, Bolnick D, Brown GW, Andrews BJ, Boone C, Myers CL

Science (New York, N.Y.)2018

DOI: 10.1126/science.aao1729 PMID: 29674565

09

Reporter-Based Synthetic Genetic Array Analysis: A Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae.

Göttert H, Mattiazzi Usaj M, Rosebrock AP, Andrews BJ

Methods in molecular biology (Clifton, N.J.)2018

DOI: 10.1007/978-1-4939-7306-4_40 PMID: 29043651

10

High-Content Screening for Quantitative Cell Biology.

Mattiazzi Usaj M, Styles EB, Verster AJ, Friesen H, Boone C, Andrews BJ

Trends in cell biology2016First author

DOI: 10.1016/j.tcb.2016.03.008 PMID: 27118708

A Dual Camera Acquisition-Spinning Disc Confocal Microscope System to Study Cellular Dynamics

Co-investigatorNSERC2023Research Tools and Instruments

Principal investigators: Botelho, Roberto

$150,000

Quantitative dissection of sources of phenotypic heterogeneity

Principal investigatorNSERC2021Discovery Grants Program - Individual

Principal investigators: Mattiazzi Usaj, Mojca

Keywords: phenotypic heterogeneity; single-cell analysis; high-content imaging; systems biology; phenotype penetrance; quantitative cell biology; interaction netrowks; subcellular morphology; yeast genetics

$164,000

Quantitative dissection of sources of phenotypic heterogeneity

Principal investigatorNSERC2021Discovery Launch Supplement

Principal investigators: Mattiazzi Usaj, Mojca

$12,500

From CIHR, NSERC and SSHRC funding decisions: CIHR since 2008, NSERC since 1991 and SSHRC since 1998, including their latest published competition results.

Frequent collaborators

Chemistry and Biology
Donnelly Centre
Molecular Genetics
Rheumatology
Biochemistry
Genetics and Genome Biology
Molecular Medicine
Other

Co-authors at Toronto Metropolitan University, colored by department. Thicker lines mean more shared papers; select anyone to open their profile and their own map.

Charles Boone

Donnelly Centre

5 shared papers, latest 2024

Brenda Andrews

Molecular Genetics

2 shared papers, latest 2024

Igor Stagljar

Biochemistry

1 shared papers, latest 2014

Igor Jurisica

Rheumatology

1 shared papers, latest 2014

Amy Caudy

Faculty

1 shared papers, latest 2018

Ming-Sound Tsao

Department of Laboratory Medicine and Pathobiology

1 shared papers, latest 2014

Jason Moffat

Genetics and Genome Biology

1 shared papers, latest 2014

Ji-Young Youn

Molecular Medicine

1 shared papers, latest 2013

Jimmy Ba

Department of Computer Science

1 shared papers, latest 2024

Quaid Morris

Faculty of Medicine / Computer Science

1 shared papers, latest 2020

Write to this professor

A short, specific email works best. This draft uses one of their recent papers; replace the parts in brackets with your own details before sending.

Applying for

Paper to mentionMention their NSERC grant from 2021

Subject: Prospective PhD student: high-content imaging Dear Professor Mattiazzi Usaj, My name is [your name], and I am a [your current degree or role] at [your institution]. I am applying for a PhD position starting in [term and year]. I read your paper "Vacuolar pH regulates clathrin-mediated endocytosis through TORC1 signaling during replicative aging." (2025), and it connects closely to my own work on [your research topic]. [One or two sentences on what you did and what you learned.] I also saw your NSERC-funded project "Quantitative dissection of sources of phenotypic heterogeneity" (2021), and I would be glad to contribute to work in that direction. Are you accepting new PhD students for [term and year]? I have attached my CV and transcripts, and I would be glad to talk at a time that suits you. Thank you for your time. Kind regards, [your name]